Bacterial growth studied by flow cytometry
نویسندگان
چکیده
منابع مشابه
Detection of bacterial aggregation by flow cytometry
Since the advent of recombinant DNA technology, bacteria have been used often to express foreign proteins [1]. Soluble proteins expressed by bacteria can be expressed either in the cytoplasm or they are secreted to the periplasm or outside the cell. In general proteins expressed in the cytoplasm are expressed to higher levels than if they are secreted[1]. Many hydrophobic and highly expressed p...
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Quantification of invasive, intracellular bacteria is critical in many areas of cellular microbiology and immunology. We describe a novel and fast approach to determine invasion of bacterial pathogens in adherent cell types such as epithelial cells or fibroblasts based on flow cytometry. Using the CEACAM-mediated uptake of Opa-expressing Neisseria gonorrhoeae as a well-characterized model of ba...
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Flow cytometry is a rapid method to quantitate live and dead bacteria in a sample.1-4 It can be used to measure the efficacy of antimicrobial compounds by exposing organisms to the disinfectant and subsequently staining with permeant and impermeant DNA dyes. Test organism viability, measured using a 30-minute flow cytometry assay, showed comparable sensitivity with a standard 48-hour plate coun...
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AIM To evaluate a flow cytometry protocol that uses reference beads for the enumeration of live and dead bacteria present in a mixture. METHODS AND RESULTS Mixtures of live and dead Escherichia coli with live:dead concentration ratios varying from 0 to 100% were prepared. These samples were stained using SYTO 9 and propidium iodide and 6-μm reference beads were added. Bacteria present in live...
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Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare hematologic malignancy with aggressive clinical course and poor prognosis. Diagnosis is based on detection of CD4+ CD56+, CD123high, TCL-1+, and blood dendritic cell antigen-2/CD303+ blasts, together with the absence of lineage specific antigens on tumour cells. In this report we present a case of BPDCN presenting with extramedullar...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1980
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990010108